Journal: Frontiers in Cellular and Infection Microbiology

Article Title: MHC Class II Activation and Interferon-γ Mediate the Inhibition of Neutrophils and Eosinophils by Staphylococcal Enterotoxin Type A (SEA)

doi: 10.3389/fcimb.2017.00518

Figure Lengend Snippet: Inhibitory effects of Staphylococcal enterotoxin A (SEA) on IL-8-induced bone marrow (BM) neutrophil adhesion (A–D) and chemotaxis (E,F) . Neutrophils were incubated with SEA (1–30 ng/ml) or Iscove's modified Dulbecco's medium (Control) for 0.5 to 4 h, and placed to adhere on VCAM-1 or ICAM-1-coated plates or allowed to migrate in 96-multiwell chemotaxis chamber. Cells were stimulated or not with IL-8 (200 ng/ml). BM neutrophil adhesion and chemotaxis were quantified by MPO measurement. Results are mean values ± S.E.M from 5 mice for each group. * p < 0.05 compared with spontaneous activity; # p < 0.05 compared with untreated cells (controls).

Article Snippet: ELISA kits for mouse IFN-γ, vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), fluorescein isothiocyanate-conjugated anti-mouse MAC-1, phycoerythrin (PE)-conjugated anti-mouse VLA-4, fluorescein isothiocyanate-conjugated anti-mouse LFA1-α and interleukin-8 (IL-8) were obtained from BD Biosciences Pharmingen (San Jose, CA, USA).

Techniques: Chemotaxis Assay, Incubation, Modification, Activity Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: MHC Class II Activation and Interferon-γ Mediate the Inhibition of Neutrophils and Eosinophils by Staphylococcal Enterotoxin Type A (SEA)

doi: 10.3389/fcimb.2017.00518

Figure Lengend Snippet: Inhibitory effects of Staphylococcal enterotoxin A (SEA) on eotaxin-induced bone marrow (BM) eosinophils adhesion (A–D) and chemotaxis (E,F) . BM eosinophils were incubated with SEA (1–30 ng/ml) or Iscove's modified Dulbecco's medium (Control) for 0.5–4 h, and placed to adhere on VCAM-1 or ICAM-1-coated plates or allowed to migrate in 96-multiwell chemotaxis chamber. Cells were stimulated or not with eotaxin (300 ng/ml). BM eosinophil adhesion and chemotaxis were quantified by EPO measurement. Results are mean values ± S.E.M from 5 mice for each group. * p < 0.05 compared with spontaneous activities; # p < 0.05 compared with untreated cells.

Article Snippet: ELISA kits for mouse IFN-γ, vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), fluorescein isothiocyanate-conjugated anti-mouse MAC-1, phycoerythrin (PE)-conjugated anti-mouse VLA-4, fluorescein isothiocyanate-conjugated anti-mouse LFA1-α and interleukin-8 (IL-8) were obtained from BD Biosciences Pharmingen (San Jose, CA, USA).

Techniques: Chemotaxis Assay, Incubation, Modification

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: MHC Class II Activation and Interferon-γ Mediate the Inhibition of Neutrophils and Eosinophils by Staphylococcal Enterotoxin Type A (SEA)

doi: 10.3389/fcimb.2017.00518

Figure Lengend Snippet: Effect of anti-MHC class II antibody on SEA-mediated inhibition of bone marrow (BM) neutrophil adhesion and chemotaxis. Neutrophils were incubated with anti-MHC class II antibody (2 μg/ml; 30 min) before addition of SEA (10 ng/ml, 2 h A,B ; 30 ng/ml, 2 h C ). IL-8 (200 ng/ml)-activated neutrophils were placed to adhere on VCAM-1 or ICAM-1-coated plates or allowed to migrate in 96-multiwell chemotaxis chamber. Neutrophil adhesion and chemotaxis were revealed by MPO measurements. Results are mean values ± S.E.M from 5 mice for each group. * p < 0.05 compared with spontaneous activities; # p < 0.05 compared with untreated cells (controls); ω p < 0.05 compared with anti-MHC class II antibody in untreated cells (absence of SEA).

Article Snippet: ELISA kits for mouse IFN-γ, vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), fluorescein isothiocyanate-conjugated anti-mouse MAC-1, phycoerythrin (PE)-conjugated anti-mouse VLA-4, fluorescein isothiocyanate-conjugated anti-mouse LFA1-α and interleukin-8 (IL-8) were obtained from BD Biosciences Pharmingen (San Jose, CA, USA).

Techniques: Inhibition, Chemotaxis Assay, Incubation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: MHC Class II Activation and Interferon-γ Mediate the Inhibition of Neutrophils and Eosinophils by Staphylococcal Enterotoxin Type A (SEA)

doi: 10.3389/fcimb.2017.00518

Figure Lengend Snippet: Effect of anti-MHC class II antibody on SEA-mediated inhibition on bone marrow (BM) eosinophil adhesion and chemotaxis. Eosinophils were incubated with anti-MHC class II antibody (2 μg/ml; 30 min) before addition of SEA (10 ng/ml, 2 h A,B ; 30 ng/ml, 2 h; C ). Eotaxin (300 ng/ml)-activated cells were placed to adhere on VCAM-1 or ICAM-1-coated plates or allowed to migrate in 96-multiwell chemotaxis chamber. Eosinophil adhesion and chemotaxis were revealed by EPO measurement. Results are mean values ± S.E.M from 5 mice for each group. * p < 0.05 compared with spontaneous activities; # p < 0.05 compared with untreated cells (controls); ω p < 0.05 compared with anti-MHC class II antibody in untreated cells (absence of SEA).

Article Snippet: ELISA kits for mouse IFN-γ, vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), fluorescein isothiocyanate-conjugated anti-mouse MAC-1, phycoerythrin (PE)-conjugated anti-mouse VLA-4, fluorescein isothiocyanate-conjugated anti-mouse LFA1-α and interleukin-8 (IL-8) were obtained from BD Biosciences Pharmingen (San Jose, CA, USA).

Techniques: Inhibition, Chemotaxis Assay, Incubation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: MHC Class II Activation and Interferon-γ Mediate the Inhibition of Neutrophils and Eosinophils by Staphylococcal Enterotoxin Type A (SEA)

doi: 10.3389/fcimb.2017.00518

Figure Lengend Snippet: Inhibitory effects of interferon-γ (IFN-γ) on IL-8-induced bone marrow (BM) neutrophils adhesion and/or chemotaxis and reversal by anti-MHC class II antibody. Neutrophils were incubated with anti-MHC class II antibody (2 μg/ml; 30 min) before addition of IFN-γ (10 ng/ml, 2 h). IL-8 (200 ng/ml)-activated neutrophils were placed to adhere on VCAM-1 or ICAM-1-coated plates (A–B) or allowed to migrate in 96-multiwell chemotaxis chamber (C) . Neutrophil adhesion and chemotaxis were revealed by MPO measurements. Results are mean values ± S.E.M from five mice for each group. * p < 0.05 compared with activities; # p < 0.05 compared with untreated cells (controls); ω p < 0.05 compared with anti-MHC class II antibody in untreated cells (absence of IFN-γ).

Article Snippet: ELISA kits for mouse IFN-γ, vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), fluorescein isothiocyanate-conjugated anti-mouse MAC-1, phycoerythrin (PE)-conjugated anti-mouse VLA-4, fluorescein isothiocyanate-conjugated anti-mouse LFA1-α and interleukin-8 (IL-8) were obtained from BD Biosciences Pharmingen (San Jose, CA, USA).

Techniques: Chemotaxis Assay, Incubation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: MHC Class II Activation and Interferon-γ Mediate the Inhibition of Neutrophils and Eosinophils by Staphylococcal Enterotoxin Type A (SEA)

doi: 10.3389/fcimb.2017.00518

Figure Lengend Snippet: Inhibitory effects of interferon-γ (IFN-γ) on eotaxin-induced bone marrow (BM) eosinophils adhesion and/or chemotaxis and reversal by anti-MHC class II antibody. Eosinophils were previous incubated with anti-MHC class II antibody (2 μg/ml; 30 min) before addition of IFN-γ (10 ng/ml, 2 h). Eotaxin (300 ng/ml)-activated cells were placed to adhere on VCAM-1 or ICAM-1-coated plates (A–B) or allowed to migrate in 96-multiwell chemotaxis chamber ( C ). Eosinophil adhesion and chemotaxis were revealed by EPO measurement. Results are mean values ± S.E.M from five mice for each group. * p < 0.05 compared with spontaneous activities; # p < 0.05 compared with (controls) and ω p < 0.05 compared with anti-MHC class II antibody in untreated cells (absence of IFN-γ).

Article Snippet: ELISA kits for mouse IFN-γ, vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), fluorescein isothiocyanate-conjugated anti-mouse MAC-1, phycoerythrin (PE)-conjugated anti-mouse VLA-4, fluorescein isothiocyanate-conjugated anti-mouse LFA1-α and interleukin-8 (IL-8) were obtained from BD Biosciences Pharmingen (San Jose, CA, USA).

Techniques: Chemotaxis Assay, Incubation